The collection is arranged into eight series. The first series contains published textual records, the second series contains non-published textual records, the third series contains records from various two-spirited organizations and gatherings, the fourth series is comprised of graphic material, the fifth series consists of a scrapbook composed of photographs and news clippings, the sixth series includes photographs, and published and non-published research on traditional Aboriginal clothing and adornments, the seventh series consists of four films, and the eighth series consists of a CBC News Manitoba video news item.

Series 1: The first series consists of published textual materials related to the Two-Spirited Movement, including books, journals and magazines, newsletters from two-spirited organizations, reports and pamphlets on AIDS, reports and articles on the Two-Spirited Movement, and newspaper clippings. Materials were published predominately from 1990 to 1996 and were created in both the United States and Canada, but were predominately created in Manitoba.

Series 2: The second series contains non-published textual records related to the Two-Spirit Movement, including obituaries and memorial service programs for two-Spirited Manitobans, drafts of poems written by a number of two-spirited individuals, and correspondence mainly addressed to Albert McLeod or the organizations he co-founded and pertaining to the creation or description of Two-Spirited organizations. The materials were created from 1988-2008, but predominately from 1990 -1996.

Series 3: The third series contains records from various two-spirited organizations and gatherings in North America, including programs, budgets, and notices of gatherings and conferences. As well as mandates, meeting minutes and job postings of many two-spirited organizations.

Series 4: The fourth series is comprised of graphic material including 9 photographs, 3 prints and drawings and 8 posters. Much of the photographs are printed in colour on computer paper and show two-spirited people at gatherings, likely held in either Vancouver or Manitoba. Many of the posters display colour photographs, and are likely from a 2007 campaign promoting condom use among two-spirited people, while others advertise gatherings. The series also includes 330 images on DVD of the 22nd Annual Two-Spirit Gathering 2010, three t-shirts and one embroidery. As well as, two ribbons and a painted rock from the first Truth and Reconciliation Commission sharing circle in 2010.

Series 5: The fifth series is comprised the contents of a scrapbook; it contains a variety of both graphic and textual materials, including 151 photographs, as well as newspaper and magazine clippings, gathering notices, and poetry related to the Two-Spirit Movement in Manitoba and across North America. The photographs consist mostly of unidentified people related to the Two-Spirit Movement in Manitoba, but also two-spirited gatherings like the Basket and the Bow, Spirituality in the ‘90’s, and Two-Spirits and HIV in New York City. Items contained in the scrapbook predominately date from 1988 to 1993.

Series 6: The sixth series is comprised of both graphic and textual materials in a variety of formats related to textile and clothing research. Graphic materials include 7 photographs, 14 pages of photocopied drawings, 1 painting and 1 magazine clipping. Textual material includes drafts and notes on Aboriginal clothing and adornment by Albert McLeod written in 1988, and photocopies of published articles.

Series 7: The seventh series consists of four films. Produced in 1990 by Mona Smith for the Minnesota American Indian AIDS Task Force, Honored by the Moon is a documentary on gay and lesbian aboriginal peoples and their place within their community historically and presently. It contains footage of interviews and gatherings. Also included in this series is unedited film from Manitoba Aboriginal AIDS Conference in 1992, PTAM Dress Rehearsal Shoot in the early 1990s, and Passing of the Legacy in Vancouver in July 2002.

Series 8: The seventh series consists of a DVD copy of a CBC Manitoba news item dated August 2, 1987 and titled "Gay Pride March." Depicts footage of the first Winnipeg Gay Pride March including shots of Albert McLeod.

The Kally Kennedy fonds includes a newspaper article (1985) and photographs (1986).

The Kerry McShane fonds includes biographical information (1985 to 1986).

The Roy Matas fonds includes obituaries (1986).

The Lawrence Adams fonds includes newspaper and magazine articles (1986-2003) and obituaries (2003-2004).

The Barbara Karinska fonds includes a biography [1988].

The Kerrie Souster fonds includes the following series: biographical information (1991-2001); newspaper articles (1988-2001); and photographs (1994-2001).

This collections contains a photocopy of a journal written in 1919 by a young woman, Katharina Hildebrandt (1901-1920), who was engaged to be married to Johann Isaac Warkentin, who was murdered on 26 October 1919 in Eichenfeld, South Russia. The collection also includes a Hildebrandt family register of birth and death dates of the children of Heinrich David Hildebrandt (1870-1919), father of Katharina, who also was murdered at the Eichenfeld massacre on October 26, 1919. Another item is a 1988 transcript of an interview of Isaac Warkentin (1908), a brother of Johann who died in 1919.

The Dianne I. Hall fonds includes a newspaper clipping (1989).

The fonds consists of material regarding women and gender issues in the University of Winnipeg, particularly around sexual harassment, the development of a sexual harassment policy at the University of Winnipeg, and related meeting minutes of the Board of Regents, the highest governance body at the University of Winnipeg. In addition to the minutes and agendas, the fonds includes publications, reports, pamphlets, and newsclippings pertaining to issues such as the status of women, sexual harrassment, student accessibility, and gender disparity on boards and amongst academic staff (including salary disparities).